【摘要】 目的 :研究高强度聚焦超声 (HIFU )及其联合顺铂 (PDD)对人膀胱癌细胞株 T2 4的体外效应。方法 :不同剂量 HIFU单独及联合 PDD作用于 T2 4细胞悬液后观察细胞存活率及生长增殖的变化。结果 :一定剂量的HIFU可使 T2 4细胞存活率直线下降并产生短暂的生长抑制作用 ;低剂量 HIFU (11.5 W/ cm2 × 6′)可使 PDD对 T2 4细胞抑制率增加 ;HIFU (45 0 W/ cm2 × 6′)与 PDD联合作用可使 T2 4细胞生长增殖在十天内基本停滞。结论 :在体外以空化、机械效应为主的 HIFU对 T2 4细胞具有急性致死及短暂的生长抑制作用 ;对 PDD具有增敏作用 ;联合PDD作用具有协同效应。更多还原

【Abstract】 Objective:To study the effects of high intensity focused ultrasound(HIFU) in combination with Cisplatin (Platinum dichloro diamine PDD) on human bladder cancer cell. Methods:After action by different intensities HIFU combined with PDD, to observe the changes of T24 cell survival rate and growth curves.Results:A certain intensity HIFU decreases the cell survival rate and inhibits cell growth of the tumor. The HIFU irradiation (11 5W/cm 2×6s) can enhance PDD inhibition to T24 cell. HIFU(450W/cm 2×6s) combined with PDD can halt T24 cell growth in 10d. Conclusion:In vitro effects of HIFU was cavitation and killing T24 cell, inhibiting cell growth and potentiating sensitivity of PDD. HIFU combined with PDD has synergitic inhibition effect to T24 cancer cell line.更多还原