【摘要】 目的 :初步探讨IL 6和IL 6R与脑胶质瘤发生发展的关系。方法 :采用逆转录聚合酶链反应 (RT PCR)方法检测白细胞介素 6 (IL 6 )和IL 6受体 (IL 6R)基因在脑胶质瘤组织标本和正常人脑胶质细胞中的表达。结果 :30例脑胶质瘤组织标本中有 2 4例 (80 0 % )表达IL 6、2 6例 (86 7% )表达IL 6R、2 2例 (73 3% )同时表达IL 6和IL 6R。人脑正常胶质细胞仅有IL 6基因弱表达 ,而无IL 6R表达。结论 :脑胶质瘤可能存在与肿瘤的恶性增殖有关的IL 6 /IL 6R自分泌或旁分泌环路更多还原

【Abstract】 Purpose:To discuss the role of interleukin 6 (IL 6) and IL 6 receptor (IL 6R) in gliomas. Methods:We detected IL 6 and IL 6R gene in 30 human glioma tissue specimens and adult normal astrocytes. A complementary DNA copy of total RNA was synthesized and amplified with specific primers using the reverse transcription polymerase chain reaction (RT PCR) method. Results:IL 6 gene was positive of in 24 (80.0%) samples of gliomas, while IL 6R gene was positive of in 26 (86.7%) cases. Co expression of IL 6 and IL 6R was identified in 22 (73.3%) specimens of gliomas. On the other hand,the expression of IL 6 was weakly positive in adult normal astrocyte, but IL 6R was negative.Conclusions:Our results indicate that IL 6/IL 6R autocrine or paracrine loop may exist in glioma, which could enhance the glioma cell proliferation.更多还原