【摘要】 目的 :探讨胃癌相关抗原与胃癌患者自身细胞毒T细胞 (CTL)细胞增殖及体外杀瘤活性的关系。方法 :用生化方法提取胃癌相关抗原 ,体外协同IL 2培养淋巴细胞 ,MTT法测定细胞增殖及杀瘤活性 ,流式细胞仪 (FCM)检测细胞表型变化。结果 :12例胃癌相关抗原阳性患者CTL细胞增殖快于 9例阴性患者 ,统计比较 ,差异有极显著意义 (P <0 0 0 1)。E/T为 80∶1时 ,前者体外杀瘤活性均值也大于后者 (P <0 0 1) ,FCM检测结果表明前者CD+ 8及CD+ 2 5表达率也显著高于后者 (P <0 0 1)。结论 :本研究为解释胃癌等“不敏感群体”现象提供实验依据 ,提示该类抗原诱导的杀伤细胞可望用于胃癌临床免疫治疗 ;结合相关抗原筛查 ,可实施个体化免疫治疗更多还原

【Abstract】 Purpose:To study the relation between TAA of gastric carcinoma and its CTL proliferation.Methods:Cytolytic cytotoxicity of autologous CTL cells were measured.TAA extracted with biochemical procedure,CTL cultured with TAA and IL 2, the proliferation and cytolytic unit evaluated by MTT assay and the phenotype changes of lymphocytes analysed by FCM.Results:CTL cells in the TAA positive group showed higher proliferation than those in the TAA negative group,with a very significant difference ( P <0 001). Compared with the TAA negative group,much higher cytolytic activity was observed in the positive group ( P <0 01). The phenotype measurement also demonstrated that CD + 8 and CD + 25 expression were enhanced significantly in the TAA positive group ( P <0 01). Conclusions:The study offers expreimental data for explaining the phenomena “insensitive group in AIT ( adoptive immunotherapy)”. The results indicate that killer cells induced with TAA and IL 2 could be effective in immunotherapy against gastric carcinoma.Morever, individual immunotherapy could be performed through the TAA screening method.更多还原