【摘要】 目的 :观察羟基磷灰石 +骨形成蛋白 +纤维蛋白粘合剂 (HA +BMP +FS)复合物增高牙槽嵴的新骨形成反应。方法 :从小牛长骨中提取骨形成蛋白 (BMP) ,首次采用羟基磷灰石 (HA)与纤维蛋白粘合剂 (FS)以及具有高效骨诱导性的BMP三者复合 ,形成一种功能互补的新的人工骨材料 ,用以进行增高犬的无牙颌牙槽嵴 ,术后 3、6、12、2 4周动物处死取标本作大体、组织学观察、组织学新骨形成量计算、四环素荧光标记显微法、扫描电镜形貌观察、背反射电子图象和X线能谱分析法等检查。结果 :发现 :(1)实验组术后 3周起出现编织骨 ,6周新骨增加 ,12～ 2 4周有成熟的骨组织相互连结 ,布满HA微粒间隙。对照组术后 3周未见新骨 ,6周个别HA微粒与基骨接触 ,12～ 2 4周少许新骨长入。 (2 )新骨组织与HA直接结合 ,钙磷元素与HA和基骨接近。结论 :HA +BMP+FS复合材料可显著地促进HA -基骨界面骨性结合 ,加快再建牙槽嵴的新骨形成 ,增加新骨形成量 ,从形态和功能上大大提高再建牙槽嵴的质量。更多还原

【Abstract】 AIM: To investigate new bone formation of alveolar augmentation with hydroxylapatite(HA)+bone morphogenetic protein(BMP)+fibrin sealant FS(HA+BMP+FS) composite.METHODS:Bone morphogenetic protein was extracted from bovine cortical bone. Hydroxylapatite particles were combined with fibrin sealant and BMP, which is of high efficient osteoinduction, forming a new complex material. The alveolar augmentation was made with this composite. The samples were investigated 3, 6,12 and 24 weeks postoperatively, with gross observation, microscope, calculation of new bone formation, tetracycline fluorescent microscope, scanning electron microscope, backscattered electron image and X-ray energy analysis.RESULTS: 1. In experimental group, weave bone had been seen since 3 weeks postoperatively and more new bone 6 weeks postoperatively; remolded bones united and distributed to all spaces of HA particles 12～24 weeks postoperatively. In control group, no new bone appeared 3 weeks postoperatively, only some HA particles contacted base bone 6 weeks postoperatively, a little of new bone grew into HA spaces 12～24 weeks postoperatively. 2. New bone directly incorporated with HA. The quantity of calcium and phosphate are similar to HA and base bone. CONCLUSION:This composite in alveolar augmentation obviously promotes incorporation HA with base bone, speeds formation of new bone and adds amount of new bone, reconstructing a bony alveolar ridge.更多还原