【摘要】 目的和方法 :以离体心脏灌流法制备大鼠心肌缺氧、复氧模型 ,提取大鼠心肌mRNA并标记心肌线粒体肌酸激酶肌膜型亚基 (sMiMi -CK)基因探针 ,进行Northern杂交 ,观察不同缺氧、复氧时sMiMi -CKmRNA的变化及磷酸肌酸 (PCr)的保护作用。结果 :随着缺氧时间的延长 ,sMiMi-CK的mRNA逐渐减少 ,复氧后sMiMi-CK进一步减少 ,PCr能显著改善sMiMi-CK的表达量。结论 :PCr对缺氧、复氧的心肌具有明显的保护作用。更多还原

【Abstract】 AIM and METHODS: Rat myocardial mRNA was extracted and mitochondrial sarcomeric creatine kinase (sMiMi-CK) was used as labled probed in vitro myocardial ischemia/reperfusion rats. Northern blot was performed to determine the changes of sMiMi-CK mRNA in different periods of ischemia/reperfusion and the protective effect of phosphocreatine (PCr).RESULTS:During early ischemial (10 min) mRNA was decreased about 18 24%. After reperfusion mRNA decreased again. PCr effectively prevented the decrease of mRNA expression of sMiMi-CK. CONCLUSION:The results indicated that PCr may possess protective effect on anoxic and reoxygenated myocardium.更多还原