【摘要】 目的 :本实验在培养的大鼠心肌成纤维细胞 (Fb)和心肌细胞上观察了肾上腺髓质素 (AM)分泌和 5种因素对AM合成和分泌的调节 ,以阐明心肌组织AM的细胞来源。方法 :乳鼠心肌Fb和心肌细胞培养 ;放射免疫法测定AM的含量 ;放射配基结合法测定Fb和心肌细胞AM受体。结果 :心肌Fb和心肌细胞均可合成和分泌AM ,而且成纤维细胞分泌AM远高于心肌细胞 (P <0 .0 1)。肿瘤坏死因子α(tumornecrosisfactor,TNFα)、脂多糖 (lipopolysaccharide ,LPS)和碱性成纤维细胞生长因子 (bFGF)可刺激Fb和心肌细胞AM的合成与分泌。转移生长因子 β (transformationgrowthfactorβ ,TGFβ)和干扰素α(interferoneα ,IFNα)可抑制Fb和心肌细胞合成分泌AM。成纤维细胞存在着高亲和力和低亲和力两种结合位点 ,而心肌细胞仅存在着高亲和结合位点 ,心肌细胞AM受体Bmax值高于Fb (P <0 0 1)。结论 :心肌Fb也合成和分泌AM ,Fb和心肌细胞都存在AM受体。更多还原

【Abstract】 AIM: To elucidate the cellular source of adrenomedullin (AM) in myocardial tissue, the effects of 5 substances on secretion of AM from cultured rat fibroblasts and myocytes were examined in this study. METHODS: Myocardial fibroblast and myocyte isolation and culture; AM was measured by radioimmunoassay; AM receptors were assayed by radioactive analysis method. RESULTS:Tumor necrosis factor α (TNFα), lipopolysaccharide (LPS) and basic fibroblast growth factor (bFGF) all could stimulate AM synthesis and secretion in myocardial Fb and myocyte , and the amount of AM secreted by Fb was greater than that by myocytes, whereas transforming growth factor-β(TGFβ),and interferon-α(IFNα) suppressed in it in myocardial Fb and myocytes. Myocardial Fb has two kinds of AM binding sites, but myocyte has only one high affinity- binding site.CONCLUSIOIN:Myocardial fibroblasts could synthesize and secrete AM, and there are AM receptors in both myocardial fibroblasts and myocytes.更多还原