【摘要】 本文通过梅岭霉素 (Meilingmycin)产生菌南昌链霉菌NS 4 1 80菌株孢子对 6种抗生素敏感性测定 ,采用诱变剂EMS四种不同诱变剂量对菌株孢子进行诱变处理 ,诱变处理的孢子涂布在含致死浓度链霉素的高氏平板上。然后从抗药性突变标志菌株中进一步筛选梅岭霉素高产菌株。在 150 0多个抗药性突变株中通过初筛获得了比诱变出发菌株的产素能力提高 50 %以上菌株。通过诱变剂量分别与抗药性突变率和突变株产素产量的变势统计分析表明 ,菌株抗药性突变与产素突变密切相关 ,产素突变的EMS诱变剂量高于抗药性突变诱变剂量 ,在 0 .0 3mol/LEMS剂量作用下 ,菌株致死率为 99.4 3% ,抗药性突变率为 0 .0 4 4 0 % ,建立了梅岭霉素产生菌抗药性突变标志诱变推理性筛选模型。为南昌链霉菌高产菌种选育研究作了有益的尝试 ,并有助于其它链霉菌属的抗生素产生菌育种研究。更多还原

【Abstract】 After testing the resistance of 6 antibiotics to the strain NS 41 80 which is the Meilingmycin producer Streptomyces Nanchangensis , about 1 500 spore resistant chemicals mutants which resist to Sterptomycin were screened after the spores regenerated on the lethal media when they were treated with 4 different dosage of super mutengent EMS. We have abtained spore mutants whose yields are 50 percent higher than CK’s among all mutants in the pre screening experments. It was showed that the resistance chemical mutation of the strains had closely relation with yield mutation and the EMS dosage of the yield mutation was higher than the resistance chemical mutation’s through the statistical analysis between the dosage of mutation and the resistance chemical mutational frequency、the variate capacity of the mutants’ yield. The lethal frequency of the spores was 99.43% and the resistance chemical mutational frequency was 0.0440% , and a model of the resistance chemical mutational labelling rational selection of the strain producing Meilingmycin was established. It was a useful experiment of the study on the screening of the high yield Streptomyces nanchangensis and it would help to the study on the screening of the other Streptomyces producing antibiotics.更多还原