【摘要】 根据猪繁殖与呼吸综合征病毒 (PRRSV)美洲型膜蛋白和核衣壳蛋白基因序列 ,设计了一对含有EcoRI和BamHI酶切位点的引物 ,用RT PCR对四个流产猪场的病料进行了检测 ,扩增出约 918bp的基因片段。通过病毒分离、酶切鉴定和序列分析证实为PRRSV感染。结果说明应用所设计的引物进行RT PCR快速检测PRRS是可行的 ,为我国快速特异诊断PRRS和PRRSV强毒株的深入研究奠定了基础。更多还原

【Abstract】 Primers for RT PCR were designed on the basis of the M and N gene sequences of U. S. isolate of PRRSV. The primers have restriction endonuclease ( Eco R I and Bam H I) sites. The tissues of stillborn fetuses of affected swine were detected by RT PCR in 4 pig farms. The samples were RT PCR positive. A gene fragment about 918 bp was amplified by RT PCR, PRRSV were isolated from the tissues of stillborn fetuses. The result showed that PRRSV can be quickly diagnosed by RT PCR, which laid a basis for quick diagnosis of PRRS and further research of the PRRSV isolates of our country.更多还原