【摘要】 目的 探讨降钙素(CT)基因高度甲基化在恶性血液病中的临床意义。方法 用限制性内切酶(HPaⅡ)消化 DNA,应用多聚酶链反应(PCR)技术检测了73例恶性血液病和对照组6例正常人以及24例非恶性血液病的 CT 基因的甲基化程度。结果 85.7%(12/14)急性淋巴细胞白血病(ALL)、60%(9/15)急性非淋巴细胞白血痛(ANLL)、80%(8/10)慢性粒细胞白血病(CML)、33.3%(5/15)淋巴瘤、2例(2/5)骨髓增生异常综合征(MDS)、1例(1/2)恶性组织细胞病(MH)、1例(1/3)慢性淋巴细胞白血病(CLL)和1例(1/9)多发性骨髓瘤(MM)的病人出现 CT基因高度甲基化阳性。而对照组6例正常人和24例非恶性血液病无1例阳性。结论 CT 基因高度甲基化可作为恶性血液病恶性克隆增殖的分子基因标志,为恶性血液病的诊断、微小残留病的监测及预示疾病的发展开辟了一条新途径。更多还原

【Abstract】 Objective To investigate the clinical significance of hypermethylation of the calcitonin(CT)gene in malignant hematological disorders.Methods Polymerase chain reaction in combination withdigestion of DNA with Hpa Ⅱ was used to examine the methylation patterns of the CT gene in 73 cases with ma-lignant hematological disorders,6 normal controls and 24 cases with nonmalignant hematological disorders.Results Hypermethylation of the CT gene occrued in 85.7%(12 of 14)patients with acute lymphoblasticleukemia,60%(9 of 15)acute nonlymphocytic leukemia,80%(8 of 10)chronic granulocytic leukemia, 33.3%(5 of 15)lymphoma,2 cases(2 of 5)with mycelodysplastic syndrome,1 case(1 of 2)with ma-lignant histiocytosis,1 case(1 of 3)with chronic lymphocytic leukemia and 1 case(1 of 9)with multiple my-eloma.However,it didn’t occured in normal controls and nonmaligmant hematological disorders.Conclu-sion Hypermethylation of CT gene may Provide an important molecular maker for tumorous cell colony andmay prove useful in diagnosing malignant hematological discorders,monitoring minimal residual disease andpredicting prognosis.更多还原