【摘要】 采用激光拉曼光谱技术研究脱氧核糖核酸 ( DNA)的热变性。实验结果表明 ,DNA热变性可以导致 :1)DNA中脱氧核糖磷酸主链振动减弱 ,包括在 884～ 890 cm-1、1112 cm-1和 1168cm-1处的谱带 ;2 )脱氧核糖 ( d)以及d中 C O的伸缩振动增强 ,在 1436cm-1和 10 72 cm-1处出现了新的谱带 ,在 1466cm-1和 10 30 cm-1处的谱带强度增强 ;3)各碱基的振动频率和相对强度都发生了很大的变化 ,碱基中羰基的伸缩振动显著增强 ,在 1650 cm-1和1670 cm-1处出现了新的谱带 ,在 1710 cm-1处的谱带强度显著增强更多还原

【Abstract】 Studying on heatdenaturation of deoxyribonucleic acid(DNA) by laser Raman spectroscopy.The experimental results show that DNA heatdenaturation can result in : 1) the deoxyribosephosphate backbone vibrations in DNA decreasing,these bands are at 884～890cm -1 、1112cm -1 and 1168cm -1 ,2)deoxyribose(d) and CO stretch vibration in d increasing,there are new bands occurring at 1436cm -1 and 1072cm -1 ,the intensities of these bands at 1466cm -1 and 1030cm -1 increase;3) Raman bands arising from all four bases undergoing very large changes in frequencies and relative intensities, carbonyl stretch vibration in bases increasing markedly,there are new bands appearing at 1650cm -1 and 1670cm -1 ,the intensity of the band at 1710cm -1 increases markedly.更多还原