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一种分离葡萄糖氧化酶的方法

A Method for Separation of Glucose Oxidase

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【作者】 戴国亮郎爱东李津如江龙

【Author】 DAI Guo Liang, LANG Ai Dong, LI Jin Ru, JIANG Long * (Center of Molecular Science,Institute of Chemistry, Chinese Academy of Sciences,Beijing 100080)

【机构】 中国科学院化学研究所分子科学中心!北京100080

【Abstract】 A method for separation of glucose oxidase(GOD) has been developed. A chroma tographic column is filled with an anion exchanger of styrene divinyl benzene matrix conta ining quaternary ammonium salt. The buffered phosphate and acetate solutions were used as eluents. Two GOD components(GOD A and GOD B) were separated by this method. Their enzyme activity and ζ potential values were 86 9, 27 3, and -4 3 mV, -6 4 mV, respecti vely . The simplicity and low cost of the method make it valuable for practical applications.

【关键词】 葡萄糖氧化酶分离活性构象
【Key words】 glucose oxidaseseparationenzymatic activity
【基金】 国家自然科学基金资助课题! ( 2 95 73 13 9)
  • 【文献出处】 应用化学 ,CHINESE JOURNAL OF APPLIED CHEMISTRY , 编辑部邮箱 ,2000年02期
  • 【分类号】O652.6
  • 【被引频次】2
  • 【下载频次】167
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