【摘要】 用中麻黄幼苗的 3种外植体进行了离体培养 .不同外植体诱导愈伤组织的结果表明 :最佳培养基为MS +2mg/L 2 ,4 D +1mg/L 6 BA ;最佳外植体为下胚轴 ;用源于下胚轴的愈伤组织进行悬浮培养 ,从第 3代开始建立起稳定的悬浮系 .愈伤组织、悬浮培养细胞及培养液中都含有麻黄碱 ,悬浮培养液中的麻黄碱含量高于愈伤组织和悬浮培养细胞 .表 2参 12更多还原

【Abstract】 Different explants of Ephedra intermedia Schrenket seedling were cultured on the media of different plant hormone combinations. The results indicated that MS+2 mg/L ?2,4 D+1 mg/L 6 BA medium was the best to induce callus, with the highest induction frequency from hypocotyl. The calli from hypocotyl were cultured in liquid medium and stable suspension culture system was established after 3 successive transfer cultures. Alkaloid components were found in the calli induced from hypocotyl, suspension cells and liquid culture medium. The content of ephedine in liquid medium was higher than those in calli and suspension cells. Tab 2, Ref 12更多还原