【摘要】 目的　从萝科鹅绒藤属植物耳叶牛皮消 (CynanchumauriculatumRoyleexWight)块根中分离出有细胞毒活性的 2种C2 1甾体苷。方法　用柱色谱和HPLC法分离纯化 ,通过化学降解及光谱分析 (UV ,IR ,1HNMR ,13 CNMR ,DEPT ,HR FAB MS ,FAB MS ,HMQC和HMBC)鉴定其化学结构。结果　从白首乌中分离得到白首乌新苷A(I)与B(II) ,其化学结构分别为 :告达庭 3 O β D 葡吡喃糖基 (1→ 4) β D 磁麻吡喃糖基 (1→ 4) β D 夹竹桃吡喃糖基 (1→ 4) β D 磁麻吡喃糖苷 ;告达庭 3 O β D 葡吡喃糖基 (1→ 4) β D 夹竹桃吡喃糖基 (1→ 4) β D 洋地黄毒吡喃糖基 (1→ 4) β D 磁麻吡喃糖苷。 结论　白首乌新苷A和B均为新C2 1甾体苷更多还原

【Abstract】 AIM To isolate and elucidate the chemical structures of two C 21 steroidal glycosides possessing cytotoxic activity from the root of Cynanchum auriculatum Royle ex Wight (Asclepiadaceae). METHODS The glycosides were isolated and purified by column chromatography on silica gel and HPLC. The molecular structures were determined on the basis of chemical evidence and extensive spectral analysis(UV, IR, 1HNMR, 13 CNMR, DEPT, HR FAB MS, FAB MS, HMQC and HMBC). RESULTS Two C 21 steroidal glycosides, named cynanauriculosides A and B, were obtained and their structures were elucidated as caudatin 3 O β D glucopyranosyl (1→4) β D cymaropynanosyl (1→4) β D oleandropyranosyl (1→4) β D cymaropynanoside and caudatin 3 O β D glucopyranosyl (1→4) β D oleandropyranosyl (1→4) β D digitoxopyranosyl (1→4) β D cymaropynanoside respectively. CONCLUSION Cynanauriculosides A and B are new compounds.更多还原