【摘要】 目的 :探讨 4种刺激剂脂多糖 (LPS)、佛波脂 (PMA)、甲酰三肽 (fMLP)和钙离子载体A2 3187及抗炎药物对HL 60细胞生成白细胞介素 8(IL 8)的作用。方法 :用ELISA方法测定IL 8的含量。结果 :LPS对 3种浓度HL 60细胞IL 8生成均有刺激作用 ,PMA ,fMLP 0 0 1,0 1,1,10nmol·L-1和A2 31870 0 1,0 1nmol·L-1亦有明显促进作用。Asp ,Sali,Hcort在 1× 10 -5mol·L-1和Iso 1× 10 -6 ～ 1× 10 -4 mol·L-1可明显抑制IL 8生成。结论 :本研究建立了可靠的筛选IL 8生成抑制剂的模型 ,表明异丹叶大黄素具有明显的IL 8生成抑制作用更多还原

【Abstract】 AIM: To study the dose effects relationship of lipopolysacchride (LPS), phorbol 12 myristate, 13 acetate(PMA), N formyl Met Leu Phe (fMLP)and calcium ionophore A 23187 (A 23187 ) on the production of intrerleukin 8 (IL 8) in cultured HL 60 cells and effects of different densities of HL 60 cells on the production of IL 8 induced by LPS. To observe the effects of several anti inflammatory drugs, such as aspirin (Asp), indomethacin (Indo), salisylic acid (Sali), meloxicam (Melo) and hydrocortisone (Hcort) on the production of IL 8 induced by LPS in HL 60 cells. METHODS: The HL 60 cells were adjusted to appropriate densities and added to tissue culture plates, then stimulants or vehicle or drugs were added. The plates were incubated at 37℃, 5% CO 2 for 24 h, then the supernatants were collected. The concentration of IL 8 in supernatants were measured by ELISA method with the limit of detection at 1 56 pg·mL -1 . RESULTS: All of the four stimulants—LPS, fMLP, PMA and A 23187 were shown to enhance the secretion of IL 8 in cultured HL 60 cells. Asp, Sali and Hcort at the concentration of 1×10 -5 mol·L -1 significantly inhibited IL 8 secretion stimulated by LPS 5 μg·mL -1 for 24 h in HL 60 cells with the inhibition ratio of 86 2, 82 77 and 136 3%, respectively. It was also inhibited by isorhapotigenin at the concentrations from 1×10 -6 to 1×10 -4 mol·L -1 , with IC 50 2 23×10 -8 mol·L -1 . CONCLUSION: An easy and trustworthy method of assay for the production of IL 8 in HL 60 cells was established, which can be used as a model of selecting inhibitors of IL 8 production.更多还原