【摘要】 目的：探讨单纯疱疹病毒胸苷激酶（HXV－TK）基因在人膀胱癌基因治疗中的作用。材料与方法：利用电击基因导入法，将逆转录病毒载体PLNXXTK导入包装细胞PA317，经G418筛选，获得稳定产病毒的PA317／PLNXXTK（PA317／TK）细胞株。采用制备的病毒液转染体外培养的人膀胱癌T24细胞，并联合丙氧鸟苷（GCV）作用，研究其对膀胱癌细胞的生长抑制作用。结果：所获得的PA317／TK细胞能稳定地产生复制缺陷的逆转录病毒载体，滴度为1．65×10~5 cfu／ml。结论：逆转录病毒介导的HXV－TK基因在体外能成功地转染T24细胞，且HXV－TK／GCV系统能明显抑制膀胱癌细胞的生长。这为HXV－TK／GCV在人类膀胱癌治疗方面的进一步体内实验提供了可靠依据。更多还原

【Abstract】 ： Purpose： In order to investigate the effect about traduction of herpes simplex virous thymidine kinase (HXV－TK) gene to treat human bladder cancer．Method： transduction of human recombinant retrovirus vector－PLNXX－TK, which contained the HXV－TK gene, into the packaging cell PA317 was been done． After selecting by using G418, the HXV－TK gene transduced cells PA317／PLNXXTK was obtained, which could produce replictation－defective retrovirus vector stably． In vitro, the retrovirus mediated HXV－TK gene was transferred into human bladder cancer cells T24 and the growth inhibiting effect by adding different dosage of GCV was observed．Result：The transferred retrovirus packaging cells PA317／PLNXXTK(PA317／TK) were obtained, which could produce replicated defective retrovirus vector and the titre of vector was 1．65×10~5 cfu／ml，and the sensitivity to GCV increased 1000 fold in HXV－TK gene transferred T24 cell compare with the untransfected contrd T24 cells．Conclusion：The retrovirus transferred HXV－TK gene can be transducted into human bladder cancer cell T24． HXV－TK gene transfection with GCV can effectively inhibit growth of human bladder cancer cell T24． The results suggest that therapy of HXV－TK gene will be a potential possibility of treatment of human bladder cancer．更多还原