【摘要】 测定脑组织细胞内 Ｃａ²⁺浓度是评价缺血引起神经元损伤的重要指标。本研究用激光扫描共聚焦显微镜（ＬＳＣＭ）和Ｆｌｕｏ－３／ＡＭ荧光探针标记技术，从单个活细胞水平检测缺血刺激诱发的海马神经元内Ｃａ２＋瞬间动态变化。结果显示低氧使胞内Ｃａ２＋浓度显著升高。谷氨酸引起Ｃａ２＋浓度升高缓慢但持续时间长。缺血对Ｃａ２＋浓度影响不显著。撤除葡萄糖则引起胞内Ｃａ２＋迅速降低。实验结果表明不同缺血刺激因素引起的钙振荡各异。用ＬＳＣＭ对活细胞内部非侵入光学断层扫描，能清晰记录到这些瞬态钙值变化。更多还原

【Abstract】 Calcium participates in many physiological and pathological metablism as a crucial signal and has important roles in ischemic neuronal damage. In the present study, laser scanning confocal microscope (LSCM) was used to measure [Ca2+]i in cultured neurons from hippocampus dyed with Fluo-3/AM. To establish intracellular calcium hippocampal neurons were exposured to hypoxia /ischemia -simulating mediums such as L-glutamate, NaCN, iodoacete, deprivation of glucose. Our data revealed the [Ca2+]i increased rapidly after adding NaCN. L-glutamate produced a persistent elevation of [Ca2+]i. We measured a detectable small elevation of [Ca2+]i after starting superfusion of ischemia-simulating medium. [Ca2+]i levels were lower in ischemia-simulat- ing medium than in the hypoxia-simulating medium. Deprivation of glucose induced a rapid decrease of [Ca2+]i, We conclude that every hypoxia /ischemia -simulating mediums produces a different change of [Ca 2 +]i.更多还原