【摘要】 目的探讨降纤酶对脑缺血再灌注损伤的保护作用及其作用机制。方法肾血管型高血压大鼠(RHR)70只 ,随机分为降纤酶组、对照组和伪手术组 ,按Longa氏方法改良复制成可再灌注的大脑中动脉闭塞 (MCAO)模型 ,缺血2h后恢复灌注。降纤酶组经股静脉给予降纤酶10U·kg-1体重 ,对照组给予等量的生理盐水。脑片行TTC及HE染色 ,观察两组在不同时间点的梗死灶的大小、脑微血管损害的表现及并发出血情况。结果降纤酶组的梗死灶的体积较对照组明显缩小 ,脑微血管损害减轻 ,镜下出血灶减少。结论降纤酶具有保护脑缺血再灌注损伤的作用。更多还原

【Abstract】 Aim To ascertain whether defibrase has the protective effect against reperfusion injury after cerebral ischemia.Methods 70 renovascular hypertensive rats(RHR) were randomly divided into defibrase group, control group and sham-operated group.Reversible middle cerebral artery occlusion(MCAO) models were produced by the modified. Longa’s method,and reperfusion was begun 2 hours after occlusion.Rats in the defibrase group were given defibrase 10 U·kg-1 body weight via femonal intraveneous injection, and in the control group with the same amount of saline. The brain pieces were processed by TTC and HE staining and the infarct size,brain microvessels damage and secondary bleeding were compared between the two groups. Results The volume of infarction in the defibrase group was obviously smaller than in the control group, the damage of brain microvessels was less severe, and the bleeding lesions under optical microscope were less than in the control group. Conclusion Defibrase has protective effect against reperfusion injury post cerebral ischemia.更多还原