【摘要】 目的探讨赤芍总甙对大鼠神经细胞脑缺血样损伤模型的保护作用及其机制。方法采用组织培养法 ,以原代大鼠大脑皮层神经细胞为材料 ,制备缺糖损伤、缺氧损伤、自由基损伤、咖啡因损伤、一氧化氮损伤及NMDA毒性损伤模型。结果形态学检查发现赤芍总甙 (totalpaeonyglycoside,TPG)对6六种脑缺血样损伤模型中的大鼠神经细胞具有明显保护作用 ,结晶紫染色也提示TPG可显著提高损伤模型中神经细胞的存活数。结论TPG对上述6种大鼠神经细胞缺血损伤均有显著的保护作用更多还原

【Abstract】 Aim To investigate protective effects and mechanism of TPG on ischemia_like injury models. Methods Six ischemia models including hypoxia, hypoglucose, oxidant injury, calcium overload, nitric oxide neurotoxicity and excitatory amino acid injury were used to assay the anti_ischemic roles of TPG in cultured primary cortex neurons. Results It was found that TPG possessed obvious protective effects on primary neurons in injury models by the way of morphological examination. Crystal violet staining also indicated that TPG increased number of life neurons in injury models significantly. Couclusions 50～200 μg·ml-1 TPG protected rat cortex cells from all injury models effectively in vitro.更多还原