【摘要】 目的　检测增生性玻璃体视网膜病变(PVR)发生过程中白细胞介素1β(IL1β)的含量变化。方法　用同种巨噬细胞诱发兔眼PVR,在不同时间点抽取玻璃体液,用双抗体夹心法酶联免疫检测(ELISA)试剂盒测定IL1β含量。结果　IL1β含量在7天时明显增加,在14～28天间呈一较高水平(175～171ng/L);21天达高峰值234ng/L,其基线含量为4ng/L。结论　IL1β在此模型的含量在7～28天明显增高,与PVR病程的炎症期、增生期和瘢痕期相吻合,提示在PVR发生中对细胞增生和瘢痕化有调控作用。更多还原

【Abstract】 ObjectiveTo investigate the concentration changes of interleukin 1β(IL 1β) in the the development of experimental proliferative vitreoretinopathy(PVR)induced by macrophages.MethodsThe cell cultured macrophage suspension was injected into the rabbits intravitreally for PVR inducement.The animals were divided into 4 groups,and were observed on 7,14,21,and*!28*!d,respectively.The criterion of this PVR model is 0,1～4 stages.The vitreous were extracted pre and post injection at the above point of times.IL 1β levels in them were measured with an enzyme linked immunosorbent assay (ELISA) kit.The data were calculated statistically.Results The PVR formation corresponded to the previous result.The trend of concentration changes in the PVR vitreous of IL 1β went up from 0*!d to 21*!d,and reached its peak 234*!ng/L at d 21,which was parallel to the inflammatory and cellular proliferative stages of PVR,then IL 1β kept the high level in the scarring stage and maintained a plateau between d 14 and d 28(175～171*!ng/L).There were no significant changes of the concentrations in vitreous and serum of the control eyes during the experiment.Conclusion Since the macrophages were the only cells induced into the rabbits’ vitreous cavity in this PVR model,all proliferative cells were derived from the hosts by themselves.And the inflammatory stage was the starting of the PVR model which may help to reveal the changes and the mediating role during the PVR formation.The increasing of IL 1β in the vitreous parallels to the inflammatory and proliferative stages and then maintained its plateau at the regenerative stage which means it may play an important role in initiating and mediating the cellular proliferation and post inflammatory regeneration in the vitreous.The data show the secretion type of cytokines in the eye belongs to the autocrine and paracrine without nervous and indocrine like regulation.更多还原