【摘要】 目的　用细胞培养法分离培养临床标本中的生殖支原体Mg ,探讨天津地区性传播疾病 (STD)门诊患者中是否存在Mg感染。 方法　采集STD门诊患者生殖道分泌物 ,接种于Vero细胞 ,进行分离培养 ,并用SP- 4培养基传代 ,对符合Mg生物学特性的阳性培养物用套式PCR、电镜观察、代谢抑制试验等方法进行鉴定。结果　在 110例STD患者中分离培养出 3株Mg ,分离率为 2 73 %。 结论　在国内首次应用细胞培养法从临床标本中分离培养到Mg菌株。从病原学证实天津地区STD患者中存在Mg感染更多还原

【Abstract】 Objective By the isolation of M.genitalium strains from clinical specimens of STD patients with Vero cell culture to observe whether M.genitalium infection exists.Methods The clinical specimens taken from STD clinic patients were cultured in Vero cell cultures grown in Eagles MEM and subcultured in modified SP-4 medium.The positive Mg isolations were identified with PCR,electron microscope and MIT.Results A total of 3 strains of Mg were isolated from 110 clinical specimens of STD patients and the prevalence was 2 73%.Conclusions It was the first time in China to isolate Mg from clinical specimens with Vero cell culture system. Mg infection existed in STD patients in Tianjing was demonstrated by the isolation of pathogen.更多还原