【摘要】 目的 :应用分子生物学方法进行遗传流行病学研究 ,探讨MTHFR基因多态性在神经管畸形的遗传易感性中的作用。方法 :应用PCR RFLP方法检测MTHFR热敏感性基因型 ;对18个NTD核心家庭进行以父母为对照的病例对照研究,计算TDT和HHRR ;另外对31例NTD胎儿和62例正常成年人进行的成组病例对照研究 ,计算MTHFR纯合突变对NTD的比值比。结果 :核心家庭分析结果 :比值比OR=3 2 ,95 %CI=1 120～11 169 ;TDT( χ2)=5 762 ,P<0 05 ,HHRR(成组χ2)=6 727 ,P<0 05 ;胎儿MTHFR纯合突变对NTD的相对危险度OR=3 21,P<0 05。结论 :研究结果说明 ,突变等位基因在神经管畸形核心家庭中存在突变基因(T)的遗传失衡现象 ,胎儿MTHFR基因第677位核苷酸的多态性是中国人NTD遗传易感性因素之一。更多还原

【Abstract】 The objective of the study is to clarify the effect of MTHFR thermolabile polymorphism on genetic susceptibility of NTD in Chinese population.MTHFR genotypes were detected using PCR RFLP analysis; 18 NTD nuclear families were analysed as case parental control study,from which Transmitted Disequillibrium Test(TDT) and Haplotype based Haplotype Relative Risk (HHRR) were calculated; 31 NTD fetuses and 62 adult controls were analysed for calculation of OR of homozygotic MTHFR.The results are as follow:Nuclear family analysis: OR=3.2( 95%CI=1 120～11 169);TDT(paired χ2)=5 762,P<0 05,HHRR(χ2)=6 727,P<0 05;homozygotic MTHFR of fetus vs adult control: OR=3 21,P<0 05. The 677th allele of MTHFR is abnormally transmitted in NTD nuclear families.Homozygotic MTHFR of the fetus may be a genetic factor of NTD in China.更多还原