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白杨不同倍性花粉的辐射敏感性及其应用

Radiosensitivity of Different Ploidy Pollen in Poplar and its Application

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【作者】 康向阳朱之悌林惠斌

【Author】 KANG Xiang-Yang, ZHU Zhi-Ti, Lin Hui-Bin (College Of Forest Resources and Environment, Beijing Forestry University, Beijing 100083, China)

【机构】 北京林业大学森林资源与环境学院!北京100083

【摘要】 通过对白杨二倍性未减数花粉和单倍性花粉萌发率和萌发进程的观察统计,证明不同 倍性花粉对(60)COγ射线辐射的敏感性不同,其中单倍性花粉的敏感性约为二倍性花粉的 2倍。 为克服二倍性花粉在参与受精过程中萌发迟缓、竞争能力较差的问题,可利用这种花粉倍性 对辐射的敏感性差异,施加一定剂量的辐射以刺激二倍性花粉萌发,同时抑制或杀死部分单 倍性花粉,从而在一定程度上相对提高柱头上萌发和萌发较快的二倍性花粉比率。三倍体育 种结果亦证明了花粉辐射在提高三倍体得率方面应用的有效性,其中在银腺杨×毛新杨组 合中,以 1470rad处理最佳,三倍体得率达3.8%,对照未检出三倍体;而在施加 1680rad的银 腺杨×毛新杨杂交组合中,则获得了高达 12.9%的三倍体。

【Abstract】 Through investigahon on germinating rate and process of unreduced diploidy Pollen and monoploid pollen in Populus tomentosa × P. alba and P. tomentosa, it was proved that there were defferent radiosensihvities to ~60Co r-ray among different ploidy Pollen, i. e. the radiosensitivity of monoploidy Pollen was two bines higher than diploidy pollen. To overcome the problems of late germination and poor compatibility of diploidy pollen during fertilization, a certain dose of radiation can be applied to shmulating the diploidy pollen germination, meanwhile to restrain of kill some monoploidy pollen based on their different radiosensitilities. Therefore the rate of germination and faster germinating process of diploidy pollen on stigmas can be increased relatively to certain extent The efficiency of radiation was proved by breeding practice. In the hybidization of (P. alba ×p.glandulosa) × (P. tomentosa × P. bolleana), 3.8% of triploid could be produced by 1470 rad treatment while no triploid is examined in the control. 12.9% of triploids were produced by the treatment of 1680 rad from the above cross.

【基金】 国家“八五”科技攻关加强项目!85-18-01-09; 国家教委博士点基金
  • 【文献出处】 遗传学报 ,ACTA GENETICA SINICA , 编辑部邮箱 ,2000年01期
  • 【分类号】Q943;S335
  • 【被引频次】55
  • 【下载频次】175
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