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普通小麦与东方旱麦草异附加系和异代换系的选育与原位杂交检测
Isolation and Verification of Triticum aestivum-Eremopyrum orientale Addition Lines and Substitution Lines by GISH
【摘要】 旱麦草属(Eremopyrum)是用于小麦品种改良的又-潜在的植物资源。为了筛选小麦-旱麦草异附加系、异代换系,对普通小麦品种 Fukoho×东方旱麦草属间杂种的 BC2F3代材料的96粒种子进行了染色体数目的检测,共检出15粒2n=43的种子, 8粒 2n= 44的种子,进一步对以上材料进行的基因组DNA原位杂交,共鉴定出3个单体附加系,2个二体附加系,1个双单体附加,1个小麦三体单体附加,1个附加3条东方旱麦草染色体的小麦单体,在染色体数为42的个体中,检测出1个单体代换,1个双单体代换。根据BC2F3代自交品系来源的不同,初步认为由双单体附加自交比单体附加自交选择异附加系的效率高。
【Abstract】 Eremopyrum is a potential genus in wheat improvement To breed T aestivum-Er. orientale additional and substitutional lines, chromosome number of 96 BC2F3 individuals were accounted. fifteen plants with 2n=43 and eight plants with 2n=44 were checked out. GISH results of the individuals with 43 chromosomes showed that they blonged to three different monosomic addihons. Two disomic addihons, one double-monosomic addition, one trible-monosomic addition and one monosomic addition were found in the 2n=44 individuals. One double--monosomic substitution and one monosomic subshtution were obtained. We also proved that it is relatively easier to obtain addition lines in the selfing population than to obtain double-monosomic additions.
【Key words】 eremopyrum; wheat; addition; substitution; genomic in situ hybridization (GISH);
- 【文献出处】 遗传学报 ,ACTA GENETICA SINICA , 编辑部邮箱 ,2000年01期
- 【分类号】Q343
- 【被引频次】55
- 【下载频次】162