【摘要】 通过往孕鼠体内连续注入ＢｒｄＵ和小鼠胚胎细胞在含ＢｒｄＵ培养基中培养，证明小鼠胚胎细胞的ＮＯＲ活性明显地被ＢｒｄＵ所抑制；当洗去ＢｒｄＵ后，ＮＯＲ活性可逐步恢复。怀孕早中期胚胎ＮＯＲ活性被抑制引起小鼠胚胎器官发育的异常：胎儿流产和出生后幼鼠死亡的比例明显增加。出生的幼鼠眼球晶状体上皮细胞异常地出现１～３个直径平均为 ０．３μｍ的不透明颗粒。经组织切片蛋白质特异化学显色表明，这些颗粒全为蛋白质颗粒。随着晶状体上皮细胞的不断解体，这些不透明颗粒大量涌进并充满了晶状体基质，最后导致先天性失明。还对１８Ｓ＋ ２８Ｓ ｒＲＮＡ基因的转录活性与胚胎器官发育的相关性进行了初步的探讨。更多还原

【Abstract】 It is reported here that the NOR activity of mouse embryonic cells treated with BrdU in Vitro. The NOR activity could be recovered after incubating the cells in the BrdU-free medium. The inhibition of NOR activity in the early and mid-term gestation led to abnormal development as follows f the increase of fetus abortion in vivo and death rate of newborn offsprings, causing a congenital blindness in the newborn offsprings. The main reason for the blindness is a contamination of a plenty of the untransparent granules with a size of 0.3pm in diameter from the epithelial cells. The relationship between the transcription of 18S and 28S rRNA genes and the embryonic organ development is also discussed in this paper.更多还原