【摘要】 ［目的］探讨蓝斑内升压素受体１是否参与室旁核对心血管活动的调节过程．［方法］动物为 Ｗｉｓｔａｒ系雄性大鼠，用乌拉坦和氯醛糖行腹腔麻醉，在观察室旁核微量注射Ｌ－谷氨酸对血压和心率的影 响的基础上，进一步观察了蓝斑内预注升压素受体１阻断剂ＯＰＣ－２１２６８后，室旁核内微量注射Ｌ－谷氨 酸对血压和心率的影响． ［结果］室旁核微量注射 Ｌ－谷氨酸引起血压下降（３．２６＋ ０．２２）ｋＰａ，心率减慢 （２１．６９±２．２３）次／ｍｉｎ；蓝斑内预注升压素受体１阻断剂后，室旁核内注射Ｌ-谷酸引起血压下降（０，９８ ± ０． ３２）ｋＰａ，心率减慢（２．８０± １．２９）次 ／ ｍｉｎ． ［结论］ Ｌ－谷氨酸兴奋室旁核所致血压下降和心率减慢的 效应在蓝斑内预注升压素受体１阻断剂后显著减弱，提示蓝斑内升压素受体１参与室旁核调节心血管 活动的过程更多还原

【Abstract】 OBJECTIVE To investigate whether the vasopressin receptor 1 in locus coeruleus is involved in the cardiovascular regulation of paraventricular nucleus of hypothalamus. METHODS The male Wistar rats were anesthetized with mixture of urethane and chloralose (ip). The L-glutamate was microinjected into paraventricular nucleus to observe for the blood pressure and heart rate, and the effects of microinjecting the L-glutamate into paraventricular nucleus of hypothalamus on blood pressure and heart rate were further observed after microinjecting the vasopressin receptor 1 antagonist OPC-21268 (VIRA) into locus coeruleus. RESULTS Microinjection of L-glutamate into paraventricular nucleus of hypothalamus decreased the blood pressure (3. 26 ± 0. 22 ) kPa and heart rate (21. 69 ± 2. 23 ) beats/min, but again microinjection of the L-glutamate into paraventricular nucleus of hypothalamus decreased the blood pressure (0. 98 ± 0. 32) kPa and heart rate (2. 80± 1. 29) beats/min after injecting the VIRA into locus coeruleus. CONCLUSION The depressor and bradycardia effects induced by excitation of paraventricular nucleus of hypothalamus are apparently attenuated by preinjection of V1RA into locus coeruleus that indicates that vasopressin receptor 1 in locus coeruleus may be involved in the cardiovascular regulation of paraventricular nucleus.更多还原