【摘要】 目的　研究胞外信号调节激酶 1(ERK1)的激活与热诱导细胞死亡的关系。方法　在细胞中稳定表达ERK1及显性失活等位基因ERK1(ERK1-KR) ,然后用克隆形成分析法测定细胞在严重热休克时的存活能力。结果　稳定表达ERK1-KR使NIH3T3和人红白血病细胞株K5 6 2对热的敏感性增加了 10 0倍。相反 ,稳定表达野生型ERK1的NIH3T3和K5 6 2细胞对热致死有耐受性。结论　热诱导的ERK的激活可以增加细胞在严重热休克时的存活能力。白血病细胞对热的敏感性以及正常骨髓前期细胞对热或其他癌症治疗的敏感性可能是由于缺少相关的ERK的激活更多还原

【Abstract】 Objective To investigate the relationship between ERK activation and heat induced cell death. Methods The dominant negative allele of ERK1 was stably overexpressed in the cells and cellular survival assays were performed by using colony formation analysis.?Results Blocking the activity of ERK1 by stable overexpression of dominant negative allele of ERK1 renders the NIH3T3 and K562 cell (an erythroleukemia cell line) up to 100 fold more sensitive to cytotoxic effects of heat. Conversely, NIH3T3 and K562 cells stably overexpressing the wild type ERK1 develop resistance to killing by heat.?Conclusion These results suggest that increased thermal sensitivity of leukemic cells, and perhaps sensitivity of normal bone marrow progenitors to thermal stress or other cancer therapy regimens could be due to lack of pertinent activation of MAPK pathway by such stress.更多还原