【摘要】 目的　探讨检测脑脊液tau、Aβ1-4 0 、Aβ1-4 2 (4 3 ) (以下简称Aβ1-4 2 )蛋白水平及其在阿尔茨海默病 (AD)诊断方面的意义。方法　用双抗体夹心ELISA法 ,共检测了 2 2例AD、15例脑血管性痴呆 (VD)和 30例非痴呆对照(NC)脑脊液中的上述 3种蛋白 ;用简单智能量表 (MMSE)、哈金斯基缺血量表 (HIS)、日常生活能力量表 (ADL)、CT和美国神经病、语言障碍和卒中 -阿尔茨海默病和相关疾病学会标准 (NINCDS -ADRDA)等判断受测者的认知情况 ,并作为临床分组诊断的依据。结果　AD、VD及NC组脑脊液tau、Aβ1-4 2 蛋白和Aβ1-4 0 /Aβ1-4 2 比值 (简称Aβ比 )存在显著差异 (P <0 .0 1或 0 .0 5 ) ,Aβ1-4 0 在各痴呆组与NC组之间变化不显著 (P >0 .0 5 )。结论　用双抗体夹心ELISA法可以在ng/L水平上检测人脑脊液tau和Aβ蛋白 ;在AD、VD及非痴呆老年人之间 ,脑脊液tau、Aβ1-4 2 蛋白存在明显的浓度梯度 ;检测老年期痴呆者脑脊液tau、Aβ1-4 2 蛋白和Aβ比可以作为生前诊断AD的一个有参考价值的生物学指标更多还原

【Abstract】 Objective To study the levels of tau,Aβ 1-40 and Aβ 1-42(43) in cerebrospinal fluid(CSF) and their significance in the diagnosis of Alzheimer disease(AD). Methods Using a sensitive sandwich enzyme-linked immunosorbant assay(ELISA), the tau, Aβ 1-40 and Aβ 1-42(43) protein levels in CSF samples of 22 AD patients,15 cerebrovascular dementia(VD) patients and 30 age-matched non dementia controls(NC) were determined. By using MMSE,HIS,ADL,GCS,HAMD,CT and NINCDS-ADRDA, the cognitive conditions of all the subjects were estimated and based upon for making their diagnoses. Results Significant elevation of tau, decrease of Aβ 1-42(43) level and increase of the Aβ 1-40 / Aβ 1-42(43) ratio(Aβ ratio) were observed in AD and VD patients(P<0.05),especially in AD(P<0.01). There were significant differences in the tau value and the Aβ ratio between AD and VD patients(P<0.05). No difference in Aβ 1-40 value was observed among AD,VD and NC groups. Conclusion The assays of CSF tau、Aβ 1-40 and Aβ 1-42(43) proteins and the Aβ ratio can provide useful biological markers for making the diagnoses of AD and VD, especially the early stage of AD.更多还原