【摘要】 目的　用60 Coγ射线照射人离体血 ,建立人淋巴细胞微核剂量效应曲线。方法　 4名健康男性外周血样以60 Coγ射线 (0 1、0 2 5、0 5、1 0、2 0、3 0、4 0、5 0Gy ,剂量率 0 5Gy/min)照射后 ,用胞质阻断微核法 (CB)和常规微核法建立剂量效应曲线。结果　在 0～ 5 0Gy剂量范围内 ,两种方法微核率随剂量增加而增加 ,呈明确的剂量效应关系。曲线拟合表明 ,回归方程式成立 ,拟合度良好 (R2 接近 1,P <0 0 1)。结论　本研究建立的剂量效应曲线可用作辐射事故时的生物剂量估算更多还原

【Abstract】 Objective To establish the relationship curves between radiation dose and micronucleus frequencies in human lymphocytes.Methods 4 healthy human blood samples were irradiated with various doses (0.1,0.25, 0.5, 1.0, 2.0, 3.0, 4.0 and 5 0 Gy) of 60 Co γ ray at 37℃(dose rate 0.5 Gy/min) and prepared by using cytokinesis block micronucleus method and conventional method in vitro.Results Dose dependent increase of micronuclei rate were observed within the dose range of 0～5.0 Gy under two methods. The curve regressions were established with high correlation ( R 2 closing to 1, P <0.01).Conclusion The curves established by this study can be used as biological dosimeters to estimate the exposure dose in a radiation accident.更多还原