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中药厚朴中厚朴酚、和厚朴酚的毛细管区带电泳分析(英文)
Determination of Magnolol and Honokiol in Cortex Magnoliae Officinalis by Capillary Zone Electrophoresis
【摘要】 本文采用毛细管区带电泳法(CZE),以硼酸-磷酸混合体系作为背景电解质,对中药厚朴中两种具有生物活性的酚类成分(厚朴酚、和厚朴酚)进行分离。经过实验条件的优化,在0.03 mol·L-1硼酸-0.01 mol·L-1磷酸-20%乙醇(pH 10.80)缓冲液,检测波长294nm条件下,厚朴酚、和厚朴酚在12分钟内可获得基线分离。实验表明这种方法简便快速,准确可靠,采用外标法可同时对厚朴中的厚朴酚、和厚朴酚进行定量测定。
【Abstract】 Two Pharmacologically active phenols (magnolol and honokiol) isolated from the Chinese herbal drug, Cortex Magnoliae Officinalis, were separated by capillary zone electrophoresis. After a series of optimization, baseline separation of magnolol and honokiol was obtained within 12 min by using the buffer system consisting of 0.03 mol·L-1 borate and 0.01 mol·L-1 phosphate, containing 20% methanol, at pH 10.80, and by DAD detection at 294 nm. With this simple and rapid method, the contents of two phenols in Cortex Magnoliae Officinalis were determined simultaneously by external standard method.
【Key words】 Cortex Magnoliae Officinalis, Magnolol, Honokiol, Quantitation; Capillary zone electrophoresis;
- 【文献出处】 Journal of Chinese Pharmaceutical Sciences ,中国药学(英文版) , 编辑部邮箱 ,2000年02期
- 【分类号】R284
- 【被引频次】5
- 【下载频次】128