【摘要】 用发根农杆菌R10 0 0菌株感染甘薯 (渝薯 34品种 ) ,获得转化毛状根 ,从毛状根中选择出了 5个无性系 ,当毛状根培养在含有 2mg/L6 BA +1mg/L玉米素的MS培养基上诱导出了愈伤组织 ,当愈伤组织培养在含有 1mg/LKT +1mg/L6 -BA +0 5mg/LIAA的培养基上 ,3周以后获得了再生植株 .用随机扩增多态性DNA(RAPD)分子标记检测出了再生植株的多态性 .更多还原

【Abstract】 Transformed hairy roots were induced in sweet potato ( Ipomoea batatas ) by Agrobacterium rhizogenes strain R1000.Five clones were selected from hairy roots.Callus was induced when hairy roots were cultured on the medium containing 2 mg/L 6 BA and l mg/L zeatin.Plantlets were regenerated from callus in 3 weeks on the media containing l mg/L KT,l mg/L 6 BA and 0 5mg/L IAA.The polymorphism of regenerated plantlets was detected by the use of a RAPD marker.更多还原