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秋水仙碱诱导生姜多倍体的研究

IN VITRO INDUCTION OF POLYPLOIDY FROM COLCHICINE-TREATED ZINGIBER OFFICINALE ROSCSE

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【作者】 郭启高张钟灵周虹梁国鲁宋明

【Author】 GUO Qi-gao 1,ZHANG Zhong-ling 2,ZHOU Hong 2,LIANG Guo-lu 1,SONG Ming 1 * (1.Department of Horticulture, Southwest Agricultural University,Chongqing 400716,China; 2.Vegetable Department of Agricultural Bureau,Chongqing 400015,China)

【机构】 西南农业大学园艺系!重庆400716重庆市农业局蔬菜处!重庆400015

【摘要】 比较生姜在不同条件下经秋水仙碱处理的诱导效果。结果表明 :在培养基中加秋水仙碱 30mg/l处理 5天 ,可使生姜的诱变率达到 6 5 .0 % ,效果最好。涂抹法无效果。 0 .1%秋水仙碱以注射法、浸泡法、包埋法处理生姜的诱变率各为 2 7.5 % ,2 2 .5 %及 47.5 %。

【Abstract】 An experiment was made to induce polyploidy in ginger ( Zingiber officinale Roscse) with 5 methods of colchicine treatment.Best results for inducing tetraploid plantets were obtained when the explants were cultured for 5 days in a medium supplemented with colchicine at 30 mg/L,the induction frequency being 65.0%. Induction frequency was 27.5%,22.5% and 47.5%,respectively, when the leaves were stripped and the bare growth tips were treated with 0.1% colchicine by injection, soaking and embedding with adsorbent cotton.No tetraploid plantets were differentiated in the treatment of colchicine dropping.

【关键词】 生姜秋水仙碱诱导多倍体
【Key words】 Zingiber officinalecolchicineinductionpolyploid
  • 【文献出处】 西南农业大学学报 ,Journal of Southwest Agricultural University , 编辑部邮箱 ,2000年05期
  • 【分类号】S632
  • 【被引频次】109
  • 【下载频次】478
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