节点文献
秋水仙碱诱导生姜多倍体的研究
IN VITRO INDUCTION OF POLYPLOIDY FROM COLCHICINE-TREATED ZINGIBER OFFICINALE ROSCSE
【摘要】 比较生姜在不同条件下经秋水仙碱处理的诱导效果。结果表明 :在培养基中加秋水仙碱 30mg/l处理 5天 ,可使生姜的诱变率达到 6 5 .0 % ,效果最好。涂抹法无效果。 0 .1%秋水仙碱以注射法、浸泡法、包埋法处理生姜的诱变率各为 2 7.5 % ,2 2 .5 %及 47.5 %。
【Abstract】 An experiment was made to induce polyploidy in ginger ( Zingiber officinale Roscse) with 5 methods of colchicine treatment.Best results for inducing tetraploid plantets were obtained when the explants were cultured for 5 days in a medium supplemented with colchicine at 30 mg/L,the induction frequency being 65.0%. Induction frequency was 27.5%,22.5% and 47.5%,respectively, when the leaves were stripped and the bare growth tips were treated with 0.1% colchicine by injection, soaking and embedding with adsorbent cotton.No tetraploid plantets were differentiated in the treatment of colchicine dropping.
- 【文献出处】 西南农业大学学报 ,Journal of Southwest Agricultural University , 编辑部邮箱 ,2000年05期
- 【分类号】S632
- 【被引频次】109
- 【下载频次】478