【摘要】 将对应NDV强毒株F基因裂解位点附近编码 14个多肽的双链寡核苷酸片段克隆至融合表达载体 pGEMEX 1,构建重组质粒GEMF14a并转化大肠杆菌DE3。将GEMF14a/DE3的融合表达产物免疫兔 ,制备抗多肽抗体。该抗体与NDV强毒株F4 8E9呈ELISA强阳性反应 ,P/N >3 5～ 7 0 ,而与弱毒NDV的Ⅰ、Ⅱ、Ⅲ和Ⅳ系呈阴性反应 ,P/N <2 0 ,证明制备的F14a抗多肽抗体是特异的 ,可用于NDV强弱毒株的毒力快速鉴别。更多还原

【Abstract】 A cDNA coding for a 14 mer polypeptide of the F gene of a virulent NDV strain was cloned into the fusion expression vector pGEMEX 1 to construct a recombinant plasmid GEMF 14a .GEMF 14a was then transposed into E.Coli JM 109 /DE 3 strain.The expressed fusion product with the 14 amino acid polypeptide was used to inoculate a rabbit to prepare a anti polypeptide antibody.Using an indirect ELISA,the antibody showed a strong positive reaction with NDV virulent F 48 E 9 strain (P/N>3 5～7 0) and negative reaction with NDV avirulent Mukteswar,Hitchner B 1,F and LaSota strains (P/N<2 0).The F 14a anti polypeptide antibody could clearly distinguish strains of low virulence from a standard virulent of NDV strain and could be used for the rapid identification of NDV.更多还原