【摘要】 目的 :探讨异基因外周血干细胞移植 (alllo- PBSCT)供者经 G- CSF或 G- CSF+GM- CSF动员后 ,采集的外周血干细胞 (PBSC)移植物中的幼稚粒细胞与单个核细胞 (MNC)、CD34 +细胞及 CFU- GM之间的相关性和移植的剂量标准及临床应用效果。方法 :对 11例 allo- PBSCT供者用 G- CSF(9例 )或 G- CSF+GM- CSF(2例 )进行动员 ,于动员前及动员后 ,分别对外周血及 MNC收集物中的幼稚粒细胞、CD34 +细胞及 CFU- GM进行检测计数 ,预处理方法主要用大剂量环磷酰胺 (CTX) +全身照射 (TBI)。 结果 :动员后外周血中的幼稚粒细胞与 CD34 + 细胞及CFU- GM同步增加 ,外周血 MNC中的幼稚粒细胞数与 CD34 + 细胞数及 CFU- GM有较好的相关性。11例患者全部植活和恢复造血功能 ,并为染色体核型或血型转为供者型所证实。结论 :allo- PBSCT供者经 G- CSF或 GM- CSF动员后 ,外周血 MNC中幼稚粒细胞与 CD34 +细胞及 CFU- GM有较好的相关性 ,因此 ,MNC与幼稚粒细胞数结合可作为 allo- PBSCT的剂量标准 ,该法检测方便快速 ,稳定性好。 allo- PBSCT能迅速重建和恢复造血功能。更多还原

【Abstract】 Objective: To study the correlation among the immature granulocyte, mononuclear cells (MNC), CD 34 \++ cell and CFU GM in collected peripheral blood stem cell (PBSC) as well as the dosage standard and effect of clinical application of allogeneic PBSC transplantation (allo PBSCT) after the donors were mobilized by G CSF or G CSF + GM CSF. Methods: Eleven donors of allo PBSCT were mobilized by G CSF (9 cases) or G CSF plus GM CSF (2 cases). Before and after mobilization, measurements of the count of immature granulocyte CD 34 \++ cell and CFU GM in collected MNC and peripheral blood was performed. The conditioning regimen mainly consisted of cyclophosphamide and total body irradiation. Result: after mobilization, the immature granulocyte and CD 34 \++ cell count and CFU GM increased simultaneously in peripheral blood. The immature granulocyte count in peripheral blood MNC was well correlated to the CD 34 \++ cell and CFU GM. All patients were engrafted and got hematopoietic reconstitution, and complete donor engraftment documented by which the chromosome keryotype or blood type were changed to that of the donors. Conclusion: After the donors were mobilized by G CSF or G CSF+GM CSF, the immature granulocyte count of MNC in peripheral blood was well correlated to the CD 34 \++ cell and CFU GM, so, the combination of immature granulocyte and MNC can be the dosage standard of allo PBSCT. This method was quick, stable, and conveninent. Allo PBSCT can rapidly reconstitute hematopoiesis.更多还原