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酶催化动力学光度法快速测定蔬菜中的农药残留毒性
Rapid Determination of the Toxicity of Pesticide Residues in Vegetables by Enzyme Catalytic Dynamic Spectrophotomerty
【摘要】 本文报道了一种蔬菜中农药残留毒性的快速灵敏的检测方法 ,检测在自行研制的小型专用便携式农药残留毒性快速测定仪上进行 ,仪器通过比较特定的酶催化显色反应的动力学曲线 ,直接显示有机磷和氨基甲酸酯类农药对乙酰胆碱酶的抑制率 ,以此求得农药残留毒性水平 ,本方法对敌敌畏的检出限为 2 2× 1 0 -3 μg/mL。
【Abstract】 In this paper a rapid and sensieive method for the determination of the toxicity of pesticide residues in the vegetables was reported. This determination was carried out with a portable pesticide detector made by ourselves, Which could directly display the inhibition rate of organophosphates and carbamates pesticide to acetyl-cholinesterase (AChE),then the level of the toxicity of pesticide residues was evaltated. This method had a detection limit of 2 2×10 -3 μg/mL for the insecticide DDVP.
【关键词】 酶抑制率;
农药残毒;
有机磷;
氨基甲酸酯;
【Key words】 enzyme inhibition rate; the toxicity of pesticide residues; organophosphates; carbamates;
【Key words】 enzyme inhibition rate; the toxicity of pesticide residues; organophosphates; carbamates;
- 【文献出处】 现代科学仪器 ,MODERN SCIENTIFIC INSTRUMENTS , 编辑部邮箱 ,2000年02期
- 【分类号】TQ450.263
- 【被引频次】77
- 【下载频次】322