【摘要】 目的　研究人脐静脉内皮细胞 (HUVEC)在支架上的粘附生长的影响因素 ,寻求最佳细胞粘附条件 .方法　HUVEC培养按文献方法进行 .利用免疫组化方法 (Sp法 )检测Ⅷ因子以鉴定细胞纯度 ,分别比较细胞代数、Fibronectin、poly-L -lysine及Ca2 + 浓度对HUVEC在支架上的粘附率的影响 .结果　 (1)第 2、3、4代HUVEC在裸支架上的粘附率分别为 (2 0 .0 5± 2 .1) %、(16 .12± 4.0 2 ) %、(10 .6 1± 5 .0 1) % (n =4) ;(2 )Fibronectin(n =6 )、poly-L -lysine(n =4)处理支架 ,HUVEC的粘附率分别为 (6 4.3± 6 .19) %、(39.88± 1.31) % ;(3)与 1mM、2mM、3mM、4mM、5mMCa2 + 相对应的细胞粘附率分别为 (5 9.81± 2 .5 8) %、(6 6 .88± 3.15 ) %、(78.35± 3.43) %、(81.6 7± 1.91) %、(84.83± 1.31) % .结论　 (1)Fibronectin、poly -L -lysine可提高HUVEC在支架上的粘附 .(2 )Ca2 + 在一定剂量范围内呈剂量依赖性地提高HUVEC在Fibronectin包被支架上的粘附 .更多还原

【Abstract】 Objective To study the influential factors on the adhesive rateio of human umbilical vein endothelial cells (HUVECs) on Ni-Ti intravascular stents.To seek for the optimum cell adhesive conditions.Methods HUVECs were cultured as Jaffe did.HUVECs were identified by Ⅷ factor detecting with immunohistochemical method(SP).The influence of different generation、Fibronection、poly-L-lysine and the different concentration of Ca 2+ on the adhesive ratio of HUVEC on the stent was studied.Results (1)Adhesive ratios of the 2nd、3rd、4th generation were (20.05±2.1)%、(16.12±4.02)%、(10.61±5.01)%(n=4) separately.(2)Adhesive rations of HUVES on stents treated by Fibronectin (n=6) and poly-L-lysine(n=4) were (64.3±6.19)%、(39.88±1 31)% separatedly.(3)The adhesive ratios were (59.81±2.58)%、(66.88±3.15)%、(78.35±3.43)%、(81.67±1.91)%、(84.83±1.31)% after culture medium supplemented with 1mM、2mM、3mM、4mM、5mM Ca 2+ .Conclusions (1)Fibronectin and poly L lysine may improve the adhesive ratio of HUVECs on stents.(2)Whitin the tested concentration limit,Ca 2+ may improve the adhesivc ratio of HUVECs on stents treated by Fibronectin.更多还原