【摘要】 目的观察ＭＥＩＡ法检测总β－ＨＣＧ和ＲＩＡ法检测β－ＨＣＧ对早孕诊断、人流术后监测等疾病中的应用价值；比较两种方法的灵敏度、特异性；分析它们之间的相关性．方法收集１２１例早孕、人流术后等血清标本，分别用雅陪全自动分析仪（采用ＭＥＩＡ法）和ＲＩＡ法检测总β－ＨＣＧ和β－ＨＣＧ．结果ＭＥＩＡ法检测早孕的灵敏度为１００％，而ＲＩＡ法为８６．１％；特异性均为１００％．总β－ＨＣＣ和β－ＨＣＣ的相关性为０．８１，ｐ＜０．００１；回归方程为：β－ ＨＣＧ（μｇ／Ｌ）＝总β－ ＨＣＧ（ＩＵ／Ｌ） ×０．０００５０２＋７．９３（当总β－ ＨＣＧ＞ ２５时），ｐ＜０．００１．结论 ＭＥＩＡ法检测总β－ＨＣＧ对诊断早孕较ＲＩＡ法检测β－ＨＣＧ灵敏；在人流术后监测有利于判断手术是否彻底；总β－ＨＣＧ和β－ＨＣＧ之间有良好的相关性，可用回归方程进行预测．更多还原

【Abstract】 Objective To evaluate the clinical utility of total β-HCG testing with MEIA in pregnancy testing, artificial abortion ect. by comparing with RIA method. To compare the sensibility, speciality and correlation between RIA and MEIA. Methods Collecting 121 cases serum samples of doubtul pregnancy and artificial abortion, assaying total β-HCG with MEIA and β-HCG with RIA. Results The sensibility of diagnosing pregnancy with MEIA is 100%, with RIA is 86.1%; the speciality is 100% in diagnosing pregnancy, either with MEIA or RIA. The correlation coefficient is 0. 81 between total β-HCG and β- HCG (p < 0.001). The regression formula is: β-HCG (μg/L) = total β- HCG(IU/L). 0.000502+ 7. 93 (caution: only when total β-HCG>25), p<0.0001． Conclusions Assaying total β-HCG with MEIA is more sensitive than assaying β-HCG with RIA in diagnosing pregnancy. It is helpful in detecting change of β-HCG in artificial abortion. There is a good correlation between total β- HCG and β-HCG, we can predict one from another one with the repression formula.更多还原