【摘要】 目的 复制克隆氏病大鼠模型，观察其组织病理学变化。方法采用Ｍｏｒｒｉｓ等在１９８９年制备克隆氏病动物模型应用的造模方法，以不同剂量三硝基苯磺酸（ＴＮＢＳ）灌肠后观察大鼠症状与结肠组织病理学改变。结果 模型大鼠随ＴＮＢＳ 灌肠浓度的增加，腹泻症状更加明显；光镜观察显示较大浓度ＴＮＢＳ 灌肠组大鼠肠道有肉芽肿形成，出现克隆氏病肠道病理改变。结论 在一定剂量范围内，ＴＮＢＳ浓度越大，致炎效果越好，０．６ｍｌＴＮＢＳ（０．２５ｍｌ ５０％乙醇中加入０．６ｍｌＴＮＢＳ）浓度灌肠能导致大鼠肠道克隆氏病的发生。更多还原

【Abstract】 Objective To prepare the modal rats with clone’s disease, and observe its histophathology change. Method Applying morris method is 1989, the rats symptoms and those colin histophathology changes were observed after use of divese dosages of TNBS. Result The more TNBS the rats had, the obvious the diarrhea is. And optical telescope showed that the rats of the more TNBS group had acestoma in intestines. Conclusion in certain dosage range, the more TNBS, the better inflammation effedct; and 0. 6ml TNBS(0. 25ML50% alcohol added 0. 6ml TNBS)could lead to clone’s disease in rats intestines.更多还原