【摘要】 目的 :分析子宫内膜异位症患者中γδT细胞和天然杀伤 (NK)细胞功能的变化 ,并探讨患者腹腔液对正常人γδT细胞和NK细胞功能的影响。方法 :采用免疫荧光细胞检测及细胞毒活性检测法分析 2 2例子宫内膜异位症患者外周血、腹腔液中的γδT细胞及NK细胞的表型与功能。结果 :子宫内膜异位症 (疾病组 )患者外周血、腹腔液中CD56+细胞与正常对照组无明显差异 ,而疾病组腹腔液中γδ+T细胞分别高于外周血和正常对照组。疾病组腹腔液中的γδT细胞及NK细胞的功能低于外周血及正常对照组。疾病组的腹腔液对正常人外周血γδT细胞及NK细胞的功能具有负相调节作用。结论 :子宫内膜异位症患者的γδT细胞及NK细胞的功能低下 ,处于被抑制状态 ,提示子宫内膜异位症患者的腹腔中存在γδT细胞及NK细胞功能的抑制因子。更多还原

【Abstract】 Obective:To investigate the activities of γδT cell and natural killer cell and their roles in the development of endometriosis.Methods:The phenotypes and activities of γδT cell and natural killer cell in peripheral blood and peritoneal fluid of patients with endometriosis were studied by immunofluorescence assay and cytotoxicity assay.Results:γδT cell in peritoneal fluid of patients with endometriosis was higher than that in peripheral blood and in control group.The activities of γδT cell and natural killer cell in peritoneal fluid of patients with endometriosis were lower than those in peripheral blood and in control guoup.The peritoneal fluid of patients with endometriosis can decrease activities of γδT cell and natural killed cell in normal women.Conclusions:The peritoneal fluid of patients with endometriosis can decrease the activities of γδT cell and natural killer cell.更多还原