【摘要】 应用RAPD PCR技术研究了不同寄主和不同季节棉蚜的DNA多态性。从 2 0种随机引物中筛选出 3种引物 ,用它们的扩增结果进行Nei的遗传距离计算 ,并根据遗传距离对所研究的棉蚜种群做聚类分析。结果表明南京地区花椒上的棉蚜与其它 4种寄主上的棉蚜在DNA水平有明显的分化。聚类分析表明 ,棉蚜季节性种群可分为 3大类群 ,即春、秋季黄色型 ,春、秋季绿色型和黄色小型蚜 (伏蚜 )。而小型蚜的遗传关系与春、秋季绿色型最为接近。更多还原

【Abstract】 The RAPD\|PCR technique was used to determine the DNA polymorphism of the aphid Aphis gossypii(Glover) collected from different host plants and in different seasons. Three primers were selected from 20 primers and used for cluster analysis based on the data of Neis genetic distance (D).The results showed that the aphids on Chinese prickly ash differentiated obviously from the aphids on the other four host plants at DNA level. The seasonal population of cotton aphid might be clustered into three groups, i.e. the spring and autumn yellow colored aphids, the spring and autumn green colored aphids and the yellow dwarf form aphid in summer. However, the genetic relationship of dwarf form was more closely to the spring and autumn green colored aphid.更多还原