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带培养室的倒置显微镜下人雪旺细胞形态和运动的观察

OBSERVATION ON THE MORPHOLOGY AND MOTILITY OF HUMAN SCHWANN CELL UNDER MICROSCOPE IN VITRO

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【作者】 杨勤邱云芳汪洋沈馨亚刘才栋

【Author】 YANG Qin Q1U Yun Fang WANG YANG’ SHEN Xin Ya LIU Cai Dong(Department of Anatomy,Shanghai Medical University 200032 Key Laljoratory of Neurobiology, Shanghai Institute of Physiology, Chinese Academy of Science 200031)

【机构】 上海医科大学解剖学教研室中科院上海生理研究所神经生物学开放研究实验室上海医科大学解剖学教研室 上海 200232上海 200232上海 200031上海 200232

【摘要】 为了显示体外培养条件下三维结构上人雪旺细胞的形态和运动方式,将引产人胎儿坐骨神经的雪旺细胞培养在聚酯纤维上,用带培养室的显微镜观察。结果显示:人雪旺细胞呈梭形,绕聚酯纤维作螺旋式迁移;有的细胞同时抓住两根纤维;在相对静止期,细胞虽无位置的迁移,但有频繁的形态变化;分裂后的子细胞有形态和运动时相的不同。

【Abstract】 The purpose of present study is to demonstrate the morphology and the motility of human fetal schwann cells cultured on the three-diamentional framework in vitro. Schwann cells were cultured on the polyester fibers and were observed continuously under the microscope with the culturing setting. The results were as follows :Schwann cells were spindle shape and had bipolar processes; They migrated in a spiral fashion; one cell could connected to two fibers; Schwann cells changed their shape in the rest stage. There were different in shape and motility between the both schwann cells after mitosis.

  • 【文献出处】 细胞生物学杂志 ,Chinese Journal of Coll Biology , 编辑部邮箱 ,2000年02期
  • 【分类号】R329
  • 【被引频次】2
  • 【下载频次】112
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