【摘要】 细胞周期素与相应的细胞周期素依赖性蛋白激酶相结合,驱动着细胞通过细胞周期各时相,而细胞周期素时相性规律大多来自酵母研究或同步化细胞的分析。本研究着重于人类非同步化细胞的细胞周期素时相性规律的揭示。采用人类白血病细胞株MOLT-4,使其处于对数生长期,加以有丝分裂中期阻滞法,应用多参数流式细胞术分析人类主要细胞周期素B1、A和E。分析发现,细胞周期素B1峰值在M期,降解于M期后,细胞周期素A峰值在G₂期,降解于M期,细胞周期素E峰值在G₁晚期,降解于S期。以上结果,使我们首次在人类非同步化培养细胞展现了主要细胞周期素的时相性表达规律。更多还原

【Abstract】 Cyclins are major proteins that combined with CDKs to drive cells pass different phases of cell cycle. Most data of cyclins schedule in human came from yeast researches or human synchronized cell lines. We present here is human major cyclins schedule of asynchronized cultured cells. The major cyclins (B1,A and E) were analyzed by cyclins/DNA multiparameter flow cytometry in exponential growth or Stathmokinesis of MOLT-4 cells. The data present that cy-clin B1 expression peak is in M phase and degradation is after M phase j cyclin A expression peak is in GZ phase and degradated in M phase;cyclin E expression peak is in late Gl and degradated in S phase. Schedule of human major cyclins in asynchronized cultured cells were presented by cyclins/DNA multiparameter flow cytometry. The data of their peaks and degradation time is very useful for cell biology and oncology research.更多还原