【摘要】 应用电生理技术研究了苦皮藤素 对兴奋性突触后电位 ( EPSP)和神经 -肌肉接头兴奋性接点电位 ( EJPs)的作用。甘露醇间隙法对美洲大蠊 ( Periplaneta americana)中枢神经离体标本兴奋性突触后电位 ( EPSP)的测定表明 ,质量浓度为 0 .1 g/L的苦皮藤素 在用药后 72 min阻断了 EPSP。细胞内微电极记录法对果蝇 ( Drosophila melanogaster)运动神经 ,即腹纵肌离体标本的神经 -肌肉兴奋性接点电位 ( EJPs)的测定表明 ,0 .1 g/L的苦皮藤素 作用 1 9.5min可阻断 EJPs。更多还原

【Abstract】 Studies have been conducted on the narcotic compound Celangulin Ⅳ from \%Celastrus angulatus\% with electrophysiological technique.Using sucrose gap recordings of synaptic transmission across the cockroach cercal nerve giant fiber synapse,it was observed that the excitatory postsynapic potential (EPSP) was blocked by 0.1 g/L Celangulin Ⅳ after 72 min of medication.Neurophysiological effect of Celangulin Ⅳ was recorded on the excitatory junction potentials (EJPs) of the drosophila larval neuromuscular junction by using an intracellular microelectrode.After bath applied Celangulin Ⅳ (0.1 g/L),EJPs was blocked in 19.5 min.It showes that the Celangulin Ⅳ effected the neuromuscular junction of insect to cause narcosis.更多还原