【摘要】 目的 :探讨铅对海马 LTP的影响与海马不同亚区神经元型一氧化氮合酶 ( n NOS)变化的关系。方法 :1 5只大鼠饮用含 0 .2 %酯酸铅 3 m后 ,作为染铅组进行实验。采用原子吸收法测定血液与海马中铅含量 ;用免疫组化法研究大鼠海马不同亚区 n NOS的表达情况。结果 :染铅组大鼠血液与海马中铅含量明显高于对照组 (血铅为 1 50 .69± 52 .1 4 ng/ml比 5.1 6± 1 .1 5ng/ml;海马铅为 1 1 69.2 7± 1 68.0 6ng/g比 1 99.63± 4 3 .1 4 ng/g,P<0 .0 5) ;染铅组大鼠海马 CA1区 n NOS阳性细胞数目 ( 49.56± 6.70 )明显少于对照组 ( 64 .56± 1 2 .4 6,P<0 .0 5) ,在 CA3区无明显改变。结论 :铅对海马 LTP的影响可能与染铅后海马各区 n NOS的不同变化有关更多还原

【Abstract】 Objective:To investigate the relationship between the effects of lead on LTP in hippocampus and changes of NOS in subfields of hippocampus.Methods:15 wister rat were fed with 0 2% lead acetate for 3 months and used for high dose lead exposed rats.Lead content of blood and hippocampus were measured by atomic absorption method and immunohistochemical method were used to investigate the changes of neuronal nitric oxide synthase (nNOS) in different hippocampal subfields in lead exposed rat.Results:In compared with control group,the blood and hippocampal lead contents were significantly increased in lead exposed rats(the blood lead content was 150 69±52 14ng/ml vs 5 16±1 15ng/ml,the hippocampal lead content was 1169 27±168 06ng/g vs 199 63±43 14ng/g, P <0 05);the number of nNOS positive neurons(49 56±6 70)in CA1 of lead exposed rats was significantly decreased( P <0 05),as compard with the control(64 56±12 46),but no significant changes were found in CA3.Conclusion:The effects of lead on LTP in hippocampus might be related to the changes of nNOS in subfields of hippocampus in head exposed rats.更多还原