【摘要】 :从长白山天池水中筛选到一株低温条件下产糖的细菌。通过薄层层析、成脎反应、毛细管等速电泳以及红外光谱法确定该糖为海藻糖;经鉴定此菌株是胶样菌属中的一个新种(ColloidesSp.)定名为CB39;与已报道的产海藻糖菌株不同的是,菌株CB39能将产生的海藻糖分泌到细胞外,18℃时其培养液中海藻糖含量为2562mg/g干菌体;采用紫外诱变法筛选到一株在25℃条件下产海藻糖量为4167mg/g干菌体的高产突变株5,产糖量是同温度下野生菌的8倍。更多还原

【Abstract】 A bacterium which had the ability of producing sugar produced sugar at low temperature (18℃) was selected from the water of Tianchi in Changbai mountain. The sugar was identified to be trehalose by methods of thin\|layer chromatography, reaction of producing osazyone, capillary electrophoresis and infrared spectrum. It was also been found that the trehalose produced by this bacterium (identified to be a new species of \%Colloides\% Sp. CB39) was exocrine. At 18℃, its trehalose content in culture solution was 256mg/g dry weight of the strain. This characteristic is different from that of other strains, which had been proved to have the ability of producing trehalose. then the strain CB39 was induced by ultraviolet in order to abstain mutants which can produce trehalose in high level at usual temperature. The mutant strain 5 that could produce trehalose inhigh level at usual temperature (25℃) was selected from all the mutant strains. The value of its productivity of trehalose is 416.7 mg/g dry weight of the strain. (At the same temperature, trehalose productivity of mutant strain 5 was eight times of that of the wild type strain CB39)更多还原