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紫色非硫细菌Rhodocista属一新分离株的鉴定及其系统学研究
CHARACTERIZATION AND PHYLOGENIC ANALYSIS ON A NEW ISOLATE OF GENUS RHODOCISTA
【摘要】 从污水处理厂分离到一株紫色非硫细菌3p ,该菌株具避光性,能形成孢囊,具片层状光合内膜,在波长798 nm 处的吸收峰很低,需硫胺素和维生素B12 作生长因子。该菌株可以琥珀酸为碳源,最佳生长温度为34 ℃~41 ℃,体内未发现R 体结构,醌类的主要成分是Q9 。对菌株3p 的16SrRNA 基因的分子系统学分析结果表明,菌株3p 与世纪红篓菌( Rhodocista centenaria) 关系最近,相似性为95 % ,二者可归为同一属;与Rc.centenaria 的杂交结果表明,二者的DNA 相关性为56 % ,故可把3p 定为一个新种:北京红篓菌( Rhodocistapekinense sp .nov) .
【Abstract】 A strain of purple non sulfur bacteria was isolated from waste water plant in Beijing. It is phototaxic, and can converted the vibrioid cells into thick wall cyst. Internal synthetic membrane is present as lamellae. It has a low absorption maxima at 798?nm. and require thiamine and vitamin B 12 as growth factors. It can use succinate as carbon and energy source, but glucose can not be used. It can not grow in 3% NaCl and had Q 9 as major quinone. The 16S rRNA gene was amplified, cloned and sequenced. A phylogenic tree was constructed on the basis of the 16S rDNA sequences. It showed that the previously known member of the genus Rhodocista, Rc.centenaria, is the nearest neighbor to strain 3 p. The level of binary sequence similarity between Rc.centenaria and strain 3 p of 95%, and their phenotypic differences and genetic DNA DNA relatedness of 56%, shows they are different species of Rhodocista. A new species name, Rhodocista pekinense, was proposed for strain 3 p.
【Key words】 Purple non sulfur bacteria; Rhodocista pekinense; Rhodocista centenaria; 16S rDNA;
- 【文献出处】 微生物学报 ,ACTA MICROBIOLOGICA SINICA , 编辑部邮箱 ,2000年01期
- 【分类号】Q939.1
- 【被引频次】24
- 【下载频次】256