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应用RAPD技术分析两个双孢蘑菇遗传家系
RAPD ANALYSIS OF TWO GENETIC FAMILIES OF AGARICUS BISPORUS
【摘要】 应用RAPD技术分析两个由同工酶为遗传标记建立的典型双孢蘑菇遗传家系中各亲子菌株间的遗传相关性,结果表明,单孢同校体的RAPD图谱较异核体而言有单一化的倾向;同时,随着遗传代数的增加,杂种子代与出发异核亲本间的遗传差异逐渐增大;研究结果还进一步印证同核单孢杂交比传统的单抱、多孢筛选更易产生优势明显的杂种.
【Abstract】 Using Random Amplified Polymorphic DNA (RAPD) techinque, the genetic relationship of the strains of two genetic families of barious bisporus, which had been founded based on the genetic marker of isozyme, was examined. The results showed that there was obvious decreasing tendency of DNA amplification polymorphism in homokaryons; as the number of genetic generation increases, the genetic distance between progenise and the original arents from which bybrids derived increased; hybrids had more advantageous characters than those isolates obtained by tranditional mule-spore or single-spore screening.
- 【文献出处】 微生物学通报 ,MICROBIOLOGY , 编辑部邮箱 ,2000年02期
- 【分类号】Q933
- 【被引频次】22
- 【下载频次】84