【摘要】 通过正交设计试验，优化了黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓ　ｎｉｇｅｒ　ｍ１２）产胞外木聚糖酶的液体发酵条件，合适的产酶培养基含麦草粉３ｇ，（ＮＨ_４）_２ＳＯ_４ ０．３ｇ，ＫＨ_２ＰＯ_４ ０．１ｇ，ＭｇＳＯ_４· ７Ｈ_２Ｏ ０．０５ｇ，ＮａＣｌ０．０３９，Ｔｗｅｅｎ ８００．３ｇ，酵母膏 ０．１ｇ，微量元素（Ｂ、Ｚｎ、 Ｍｎ、 Ｆｅ、 Ｍｏ）定容１Ｌ；接种量为１．０ ×１０^７个孢子／ ５０ｍＬ培养基，２８℃，１２０ｒ／ｍｉｎ，振荡培养５ｄ，木聚糖酶活力达３４．４７ｕ／ｍＬ。更多还原

【Abstract】 Through orthogonal experiment, the optimal condition was given for producing extracellular xylanases of Aspergillus niger ml2. The suitable medium contained 3% wheat-straw powder, 0.3%（NH₄）₂SO₄, 0.1% KH₂OP₄, 0.05% MgSO₄·7H₂O,0.03% NaCl,0.3% Tween 80, 0.1% yeast extract, and trace elements （B, Zn, Mn, Fe and Mo）. 1.0 × 10₇ spores were inoculated into 50mL medium in 250mL Erlenmeyer Flask in a water rocker （120r/min,28℃） which stirred for 5.5 days. The xylanase activity of culture filtrate of ml2 was 34.47u/mL.更多还原