【摘要】 将乙型脑炎SA14 14 2减毒株 (简称 14 2株 )病毒液和灭活 14 2株病毒液皮下和腹腔免疫小白鼠 ,P3株死苗作对照 ,测免疫效价 ,结果 14 2株病毒液的效力为 1 8× 10 -5～ 6 .1× 10 -6ID50 /ml,灭活 14 2株病毒液为 9 9× 10 -1～ 7 2× 10 -3 ID50 /ml,P3株死苗为 4 0× 10 -3 ～ 2 2× 10 -4 ID50 /ml。同时免后 14天采血用PRNT法测抗体 ,结果为 14 2株病毒液 1∶2 (皮下和腹腔 ) ;灭活 14 2株病毒液 <1∶2 ,P3株死苗为皮下 1∶2、腹腔 1∶10。结果表明 :14 2株在小白鼠体内的免疫原性远远高于灭活 14 2株病毒液和P3株死疫苗 ,14 2株经灭活后对小白鼠基本无保护作用。 14 2株抗体水平不高 ,而免疫力远高于P3株死苗 ,说明 14 2株与P3株死疫苗免疫性质不同 ,有细胞免疫参与。更多还原

【Abstract】 Mice in groups were subcutaneously and peritoneally inoculated with live and inactivated attenuated SA 14 14 2 strain virus and inactivated P3 strain vaccine separately.14 days after the inoculation,the mice were bleed to test the antibody.Meanwhile the mice were challenged with P3 strain virus to test the efficacy.The efficacy was:live SA14 14 2 strain:1.8×10 -5 ～6.1×10 -6 ID 50 /ml,inactivated SA14 14 2 strain 9.9×10 -1 ～7.2×10 -3 ID 50 /ml,P3 strain vaccine 4.0×10 -3 ～2.2×10 -4 ID 50 /ml.The antibody level was:SA14 14 2 strain 1∶2(subcutaneously and peritoneally),inactivated SA14 14 2 strain<1∶2(subcutaneously and peritoneally),P3 strain vaccine 1∶2(subcutaneously),1∶10(peritoneally).The experimental results indicate:the immune efficacy of SA14 14 2 is much higher than that of inactivated SA14 14 2 and P3 strain.The antibody level of live attenuated SA14 14 2 strain was lower(compare with P3 strain),but the immune efficacy was much higher,indicates that the live attenuated SA14 14 2 strain has different mechanical immunogenecity.It might induce both humoral and cellular immunity.更多还原