【摘要】 通过分析 11β-羟基转化体系中反应介质和发酵液的影响 ,设计了一种将化合物 RS- 2 1醋酸酯转化为氢化可的松的新工艺。确定工艺条件为 :孢子接种浓度 1× 10 6/ m L ,2 8℃ ,摇床转速 (150～ 170 ) r/ min,培养 (17～ 2 1) h,培养液 p H值降至 3.8、菌体处于稳定期前期、菌丝干重达 7.2 mg/m L以上时 ,以 1∶ 1稀释比稀释 ,投料转化 2 4 h,β体转化率达 (75～ 76 ) %。初步研究了新工艺的转化过程特性更多还原

【Abstract】 WT5”BZ]According to effect of the reaction medium and cultural medium on 11β hydroxylation,the new bioconversion technology was established which Reichsterins substance acetate was converted to hydrocortisone by 11β hydroxylation.The effect facts were observed.The process conditions were:sports concentration 1×10 6/mL,temperature 28 ℃,rotation speed of shake flask (150～170) r/min,hypha age (17～21) h,pH volute of culture fluid 3.8,hypha weight (dry weight) 7.2 mg/mL,rate of dilution 1∶1,fermentation periods 24 h.The rate of conversion of hydrocortisone was 75%～76%.The 11β hydroxylation character of new technology was studied. [WT5”HZ]更多还原