【摘要】 目的:研究OK-432与IL-2联用,抑制近交系小鼠C₅₇BL/6 Lewis肺癌的作用。方法:以G₅₇BL/6小鼠Lewis肺癌为模型,观察OK-432联合IL-2对肿瘤发生、生长、转移的影响,及体内对肿瘤细胞的作用。结果:二者联用对肿瘤体积和重量的抑制作用明显增强（P<0.01）,增强抑瘤率（P<0.05）,肺转移灶的数目明显减少。光镜下显示肿瘤灶中出现许多凋亡细胞;肿瘤组织电镜观察,瘤细胞核染色质浓集成块,在核膜内呈新月形或环形排列,核膜清楚,瘤细胞内质网扩张,线粒体肿胀。结论:在二者剂量减半的情况下,联合用药的抑瘤效应仍得到加强,克服了IL-2的毒副作用,其杀瘤过程主要为对瘤细胞的直接攻击,和促进体内多种反应细胞相互作用,诱导瘤细胞的凋亡。更多还原

【Abstract】 Objective:To study the anti-tumor effect of combination OK-432 and IL-2 therapy on inbred C57BL/6 mice bearing Lewis lung cancer (LLC). Methods: As C57BL/6 mice bearing LLC model,OK-432 and IL-2 combination effects were observed on tumor occurrence, growth, metastasis, and tumor cell changes under light and electron microscopes in vivo. Results: Combination administration group significantly enhanced inhibition effects on tumor volume and weight versus control (P<0.01), increased inhibition rate(P< 0.05), and distinctly reduced the number of pulmonary metastasis foci. Under electron microscope,tumor cell emerged karyopyknosis,new-moon shaped or circular,nucleus membrane was clear,endoplas-mic reticulum was dilated,and mitochondrion was swollen. A lot of apoptosis cells emerged in the tumor foci under light microscope. Conclusion: Under the half-dose of them,combination therapy still had strengthened effects of inhibition on tumor without IL-2 ill-effect. The process of killing tumor was mainly to attack tumor cells directly,improving some kinds of cell interactions and inducing tumor cell apoptosis.更多还原